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Soma A Onodera A Sugahara J Kanai A Yachie N Tomita M Kawamura F Sekine Y 《Science (New York, N.Y.)》2007,318(5849):450-453
A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3' half of the tRNA lies upstream of the 5' half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini. 相似文献
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Sakudo A Tsenkova R Tei K Morita H Ikuta K Onodera T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1375-1378
The risk of infections from zoonotic pathogens of tissues and/or tissue-derived products has been increasing. One preventive approach in reducing infection risk is tissue decontamination, where selection and screening of highly infectious tissues are strictly followed. Therefore, the development of reliable analytical methods for rapid tissue discrimination is essentially important. In the present study, a procedure has been developed for intact tissue discrimination on the basis of multivariate analysis of visible and near-infrared (Vis-NIR) spectra of certain tissues such as brain, liver, kidney and testis of mice without any pretreatment. Transmittance spectra in the 600- to 1000-nm regions were subjected to a principal component analysis (PCA), and leave-out cross-validation was employed to develop multivariate models for tissue discrimination. The plot of PCA scores against Vis-NIR spectra of brains, kidneys, livers and testes from 11 mice portrayed reliable tissue discrimination. This result suggests that Vis-NIR spectroscopy combined with chemometrics analysis may provide a potentially useful approach for rapid non-destructive discrimination of tissues. 相似文献
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The orchestrated migration of T lymphocytes is important for generating immunity and maintaining immunological tolerance. T lymphocytes can be divided into two populations, αβ T cells and γδ T cells, on the basis of their expression of different forms of the T cell receptor (TCR). γδ T cells represent an innate subset of T lymphocytes that play an important role in early immune response against a variety of pathogens, including bacteria and viruses. γδ T cells are abundant in the epithelial tissues. In ruminants and pigs, they constitute a major proportion of the blood lymphocyte pool, unlike in rodents and humans. Although recent studies using large animals have suggested that epithelial γδ T cells are the major source of γδ T cells in peripheral blood, and that they recirculate between epithelial tissues and blood via lymphatics, the migration pattern of these cells is largely unknown. The aim of this review is to provide an overview of the current knowledge on γδ T cell migration under steady-state conditions. A deeper understanding of γδ T cell migration may enable therapeutic modulation of innate immune responses. 相似文献
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Mayu Onodera Takayuki Nakajima Masami Nanzyo Tadashi Takahashi Donghe Xu Koki Homma 《Plant Production Science》2013,16(2):262-274
ABSTRACT Soil salinity is a major constraint to sustainable crop production. Genetic improvements are needed for growing soybean in salinity-prone environments. Salt-tolerant soybean genotypes alleviate a reduction in photosynthesis and growth under saline conditions; however, the detailed mechanisms involved remain unclear. Here, we aimed to clarify how Na and Cl root-to-leaf transport is quantitatively regulated, and to identify whether photosynthetic tolerance depends on traits associated with either stomata or with mesophyll tissues. Two pairs of pot-grown soybean near-isogenic lines (NILs) consisting of tolerant and susceptible counterparts, derived from a cross between salt-tolerant FT-Abyara and salt-sensitive C01, were subjected to salinity treatment in a rainout greenhouse. Comparison of photosynthetic responses between genotypes indicated that genotypic differences in salinity tolerance depended on the ability for sustained CO2 assimilation in mesophyll tissues, rather than stomatal conductance. The ratio of photosynthetic rate to intercellular CO2 concentration (A/Ci) declined exponentially with increasing Na and Cl concentration regardless of genotype, but tolerant genotypes effectively kept both elements at significantly low levels. Under saline conditions, tolerant genotypes reduced Na and Cl content at the two transport pathways: from root to stem, and from stem to leaf, but the reduction of Cl at each pathway was only minor. These results suggest that integrating genetic capacity for Cl transport regulation and osmotic adjustment should be an important target in salinity-tolerance soybean breeding. 相似文献
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Abstract CASE HISTORY: A dog that had received 8 months of cyclosporin and ketoconazole therapy for treatment of atopic dermatitis subsequently developed severe neurological disease, that failed to respond to treatment with trimethoprim-sulphadiazine and clindamycin. HISTOPATHOLOGICAL FINDINGS: Histopathological examination of the pulmonary parenchyma and spinal cord revealed loose aggregates of Gram-positive, partially acid-fast, fine, beaded, filamentous bacteria, most consistent with Nocardia spp. DIAGNOSIS: A presumptive diagnosis was made of disseminated nocardiosis of the spinal cord and lungs. CLINICAL RELEVANCE: Nocardia spp. is an opportunistic actinomycete that may cause disseminated disease, particularly in immunocompromised animals. Cyclosporin is used in veterinary medicine to control immune-mediated and allergic disorders, with few reported adverse side effects. This case gives further evidence that involvement of the spinal cord in nocardiosis of the central nervous system (CNS) carries a poor prognosis, and opportunistic infection by Nocardia spp. may be a potential complication of immunosuppressive cyclosporin therapy in the dog. 相似文献